Search results for "Paramagnetic particles"
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Purification by affinity chromatography of H1 RNA-Binding Proteins from rat brain
2003
Post-transcriptional regulation of mRNA metabolism is involved in processes as different as cell fate specification in development and cell response to a large variety of environmental cues. Regulation of all steps of RNA metabolism depends on RNA-binding proteins (RBPs). By using a T1 RNase protection assay, we previously identified three H1° RNA-binding factors (p40, p70 and p110), highly expressed in the rat brain. Here we report enrichment of these factors from brain extracts, obtained by affinity chromatography of biotinylated H1° RNA-protein complexes on streptavidin-conjugated paramagnetic particles. The purified proteins maintain RNA-binding ability and preference for histone messag…
Bending of flexible magnetic rods.
2004
The flexible inextensible magnetic rod model is applied for the study of bending and buckling deformations of the paramagnetic particle chains linked by polymer molecules. It is shown that the existing experimental results can be reasonably well described by this model which takes into account the normal magnetic forces arising at chain bending deformation. By matching the experimentally observed shapes with our numerical simulation results different physical properties of the linked paramagnetic particle chains are determined.